Knowledge-enriched characterization of cell populations. Morphological info from photos can include to your richness of flow cytometry information. For instance, the figure reveals an otherwise typical apoptosis assay employing Annexin V and PI, including mobile imaging to characterize cells in Just about every inhabitants to reveal morphologically distinct characteristics. These insights could not are already received from multiplex staining by itself.
As samples are obtained about the Attune CytPix Flow Cytometer, the substantial-velocity brightfield camera captures and retailers illustrations or photos of detected occasions in a rate up to six,000 illustrations or photos per second, depending upon the flow charge and graphic measurement. For greater versatility, Attune Cytometric Software lets you alter the graphic capture frequency as necessary and choose a particular gate for image seize, if essential.
Combine acoustic concentrating technological innovation with superior-parameter Assessment for versatile spectral unmixing and conventional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating that has a significant-speed brightfield digicam for simultaneous large throughput flow cytometry and significant resolution brightfield imaging.
"An image is just not very well worth 1000 dots, but it genuinely helps to see visuals. It is great to discover photos that correlate with staining. For example, AnnexinV-favourable cells that are morphologically unique than healthy cells, or CD14+ monocytes that happen to be much larger plus more textured than CD3+/CD4+/CD14- lymphocytes.
Listed here, a highly skilled person has gated singlets confidently. Following analyzing the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate is made up of more than 4% aggregates.
Optimizing gating tactic. Even strong manual singlet gating is mistake-inclined and remains a subjective final decision level in Practically all flow cytometry assays. Imaging can be used to verify and regulate gates to include only one cells of curiosity.
Constant picture top quality even at substantial throughput Acoustic concentrating as well as a superior-pace digital camera Blend to image these T cells persistently at reduced or large flow charges. Quickly adjust aim and camera settings to fulfill experimental requirements.
Early signs of this contamination experienced at first been dismissed as https://myeasybookmarks.com/story4661172/flow-cytometer-a-revolutionary-tool-for-cell-analysis particles. Backgating to find out new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also helps you to use morphological functions to find appealing subpopulations that will not be apparent from flow cytometry facts by yourself.
Perhaps most significantly, these functions comprise cells of Evidently unique phenotypes which could lead to incorrect conclusions pertaining to double beneficial events (especially in exceptional populations).
AI-pushed automatic graphic analysis Together with the Attune Cytometric Application translates celebration options into unique morphology parameters which might be coupled with typical fluorescence and scatter parameters to reveal really reproducible population adjustments and unique subsets.
Software note: Automated picture Assessment decreases person-to-consumer variability in flow cytometry gating procedures
Leverage the strength of Superior technological innovation and innovation to accelerate your discoveries. Belief while in the Attune Flow Cytometer household to provide the reliability, velocity, and accuracy that established your do the job apart.
No wash/no lyse leukocyte Examination with imaging. Cells had been obtained from 24-hour-previous blood by dilution in 1 mM EDTA ( Professional UX/UI allows customers to prepare AI models for expanded applications. (A) The “Educate Design” attribute provides annotation equipment and authentic-time design coaching accuracy.
At the center of each Attune Flow Cytometer lies our groundbreaking acoustic concentrating technological know-how, which makes use of audio waves to meticulously align cells throughout the flow cell. This impressive tactic surpasses standard hydrodynamic methods, causing a lot quicker processing speeds, exceptional information excellent, and greater sample throughput.
Because the cell line experienced Formerly been analyzed within the Attune CytPix Flow Cytometer, the researchers went back again to the pictures and have been in a position to document the microbial infection no less than five times earlier.
Agilent Value Assure guarantees you at the least 10 years of instrument use from your day you purchase. If not, we will credit you Along with the residual worth of the program toward an upgraded design.
What appeared to be a homogeneous inhabitants is definitely more numerous—an insight that ought to be regarded as when interpreting results.